(A) Flow cytometry quantification of circulating Compact disc11b+ myeloid cells and Ly6Chigh monocytes entirely blood seven days following MI, n = 6C7 mice per group; (B) Quantification of LSK, HPSCs, GMPs and MPPs in the bone tissue marrow 48 h and 7 d post-MI; left: consultant FACS (fluorescent triggered cell sorting) plots; striking numbers reveal percentual percentage of total living cells, correct: quantification of Lineage?/Sca1+/cKit+ LSK, Lineage?/Sca1+/cKit+/CD48?/Compact disc150+ HPSCs, Lineage?Sca1?/cKit+/CD48?/CD150? MPPs, Lineage?Sca1?/cKit+/CD16/CD32+/CD34+ GMPs per femur; n = 7C10, (C) + (D) mRNA manifestation of Vcam-1, Cxcl-12, Angpt1 and Kitl in the bone tissue marrow (C) and spleen seven days after MI (D), n = 4C8 mice per group; mean + SEM, 1-way-ANOVA or KruskalCWallis check with Dunns multiple evaluations check
(A) Flow cytometry quantification of circulating Compact disc11b+ myeloid cells and Ly6Chigh monocytes entirely blood seven days following MI, n = 6C7 […]