The following primary antibodies were used: affinity-purified polyclonal rabbit antibodies against human Pitx2 (1:1000) as explained by Hjalt (2000) ; polyclonal antibodies to green fluorescent protein (GFP) (1:1000; 1X70 microscope having a 20 objective and digital images were acquired with WinView/32 software (Princeton Tools, Trenton, NJ) and a PentaMax KDK-1400 charge-coupled device camera (Princeton Tools)
The following primary antibodies were used: affinity-purified polyclonal rabbit antibodies against human Pitx2 (1:1000) as explained by Hjalt (2000) ; polyclonal antibodies […]